HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.
The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis.
End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups.
mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis.
mHMGCS is expressed in liver and kidney.
Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it.
A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human HMGCS1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human HMGCS1.
