High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin.
Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix.
HMG proteins synergize with Oct-2, members of the NF˚B family, ATF-2 and c-Jun to activate transcription.
Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein.
Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes.
HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human HMGB1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human HMGB1.
