PICH (Plk1-interacting checkpoint helicase), also known as DNA excision repair protein ERCC-6-like (ERCC6L) or tumor antigen BJ-HCC-15, is a 1,250 amino acid protein belonging to the SNF2/RAD54 helicase family.
PICH is a DNA helicase and an essential component of the spindle assembly checkpoint.
During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin.
PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores.
PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region.
PICH/Plk interaction is also required for correct Plk localization to the kinetochore.
PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ERCC6L.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ERCC6L.
