REDD-2 (regulated in development and DNA damage response 2), also designated Rtp801L or DDIT4L (DNA-damage-inducible transcript 4-like), is a 193 amino acid cytoplasmic protein belonging to the DDIT4 family and is predominantly expressed in skeletal muscle.
Considered a stress-inducted protein, REDD-2 is a negative regulator of the mTOR (mammalian target of rapamycin) pathway.
mTOR is a serine/threonine kinase that plays an essential role in cell growth control and is an important regulator of skeletal muscle size.
Highly expressed in human atherosclerotic lesions and macrophages, REDD-2 mediates monocyte cell death through reduction of Trx (thioredoxin-1) expression.
REDD2 expression in macrophages increases oxidized LDL (oxLDL)-induced cell death, suggesting that REDD2 may play a critical role in arterial pathology.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human DDIT4L. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DDIT4L.
