Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein.
Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end.
The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1.
Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin.
In addition, Dynactin p62 is distributed in the nucleus at very high expression levels.
Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:100-1:300 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human DCTN4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DCTN4.
