CHRDL2 (chordin-related protein 2), also known as BNF1 (breast tumor novel factor 1) or CHL2 (chordin-like 2), is a 429 amino acid protein that belongs to the chordin family of proteins.
CHRDL2 contains three VWFC (von Willebrand Factor type C) domains and is predominantly expressed in uterus and moderately expressed in prostate, liver, ovary, heart and testis.
Due to alternative splicing events, CHRDL2 exists as five isoforms, namely isoform I, isoform II, isoform VII, isoform VIII and isoform IX.
Two of these isoforms are secreted, while the other three localize to the cytoplasm.
Functioning as a BMP-binding inhibitor, CHRDL2 directly interacts with BMPs and blocks their binding to BMP receptors, thereby inhibiting BMP activity.
In breast, lung and colon tumors, CHRDL2 expression is upregulated, suggesting a possible role in tumorigenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human CHRDL2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human CHRDL2.
