TMEM16A, also known as DOG1, ORAOV2, TAOS2 or ANO1 (anoctamin 1), is a 986aa multi-pass membrane protein that localizes to both the cell membrane and the cytoplasm and belongs to the anoctamin family.
Expressed in a variety of tissues with highest expression in skeletal muscle and liver, TMEM16A functions as a calcium-activated chloride channel that is required for normal tracheal development.
Human TMEM16A shares 90% sequence identity with its mouse counterpart, suggesting a conserved role between species.
TMEM16A is present in breast, pancreatic, gastric, and uterine cancers, as well as in neck, ovarian and parathyroid tumors, suggesting a role for TMEM16A in carcinogenesis.
Three isoforms of TMEM16A exist due to alternative splicing events.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry: 1:25-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ANO1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human ANO1.
