Unlike most eukaryotic kinases, alpha kinases, such as ALPK1, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Epithelial cells maintain a polarized structure based on a selective sorting machinery for cargo traveling to the apical or the basolateral membrane domain at the trans-Golgi network exit.
Alpha-kinase 1 (ALPK1)is a compnent of raft-carrying apical vesicles, originally identified in vesicles ferrying raft-associated sucrase-isomaltase (SI).
It has been proposed that phosphorylation of myosin I by ALPK1 is essential to the apical trafficking of raft-associated SI.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:100ELISA: 1:5000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ALPK1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the N-terminal region of human ALPK1.
