The deduced full-length ACAD11 protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains.
It also has a glycosylation site and a C-terminal peroxisome-targeting signal.
The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs.
Real-time RT-PCR detected variable ACAD11 expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney.
Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11 associated with mitochondrial membranes, but not mitochondrial matrix.
Immunohistochemical analysis showed that ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry: 1:15-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ACAD11. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human ACAD11.
