Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-beta signals from the cell surface into the nucleus.
There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads.
Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their C-terminal (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I.
Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human SMAD4 at ~60kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa200-300 of human SMAD4, conjugated to KLH.
