ATG4D, Autophagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases.
Members of this family play a role in the biogenesis of autophagosomes (1).
Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy.
Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine.
Exposure of the glycine at the C-terminal is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.
Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry/Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ATG4D at ~53kD.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa98-108 of human ATG4D, at N-terminal.
