Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kD, and can be found ubiquitously throughout mammalian tissue.
LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II.
This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded.
Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes.
LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry/Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human LC3A at ~14kD. Band at 50 kDa is LC3A complex. Species Crossreactivity: rat, mouse.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-12 of human LC3A, at N-terminal.
Shelf Life：1year
Swiss Prot Number：Q9H492
EU Commodity Code：30021010
商品名：LC3A (MAP1 Light Chain 3 Like Protein 1, Map1lc3a, MAP1BLC3, Autophagy-Related Ubiquitin-Like Modifier LC3 A, MAP1LC3A, LC3A, Microtubule-Associated Proteins 1A And 1B, Light Chain 3, MAP1ALC3, MAP1A/1B Light Chain 3 A, MAP1A/MAP1B Light Chain 3 A, Microtubule-Associated Protein 1 Light Chain 3 Alpha, LC3, Microtubule-Associated Proteins 1A/1B Light Chain 3A, Autophagy-Related Protein LC3 A, MAP1A/MAP1B LC3 A, ATG8E, MLP3A HUMAN, MAP1 Light Chain 3-Like Protein 1, Microtubule Associated Proteins 1A/1B Light Chain 3) 100ug
