The Tumor Necrosis Factor Receptor (TNFR) also known as Cluster of differentiation (CD120) is a protein that belongs to the (TNF)/(TNFR) superfamily.
TNF interacts with two distinct receptors TNFR1 and TNFR2.
These receptors share no homology on their cytoplasmic sequences (1,3).
TNFR1 also known as p55/p60 is a high affinity receptor for TNF-alpha.
The TNFR1 has an extracellular domain with variable numbers of cysteine-rich repeats.
The functional properties of TNFR1 are targets in new therapies for osteoporosis, chronic inflammatory and autoimmune diseases (1, 2).
The TNF-alpha/TNFR1 receptor complex is responsible for the recruitment and the subsequent activation of the caspase (aspartate-specific cysteine proteases) that regulate apoptosis.
Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry/Immunofluorescence: 1:100Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TNF-R1 at ~55kD. Species Crossreactivity: canine, human, monkey, rat, bovine, rabbit.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Bo Ca Hu Mk Mo Rb Rt
Immunogen：Synthetic peptide corresponding to aa20-43 of mouse TNF-R1.
