84-4459-24　PDI (PDA2, PDI, PDIA2, PDIP, Pancreatic Protein Disulfide Isomerase) 100ul　384972

特徴

• The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds.
• Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1).
• Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3).
• PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5).
• PDI is an abundant 55kD protein located primarily in the ER, however studies have also proved its presence in the cytosol (1).
• PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its C-terminal (6).
• It uses C-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER.
• This retention is reported to be mediated by a KDEL receptor (7).
• Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunocytochemistry/Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.