84-4459-23　Calnexin-CT (Calnexin, CALX HUMAN, CANX, CNX, Histocompatibility Complex Class I Antigen Binding Protein p88, IP90, Major Histocompatibility Complex Class I Antigen-Binding Protein p88, P90) 200ul　384971

特徴

• Calnexin, an abundant ~90kD integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1).
• It consists of a large 50kD N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3).
• Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3).
• Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins.
• Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6).
• Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
• Applications:Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunocytochemistry/Immunofluorescence: 1:100Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.