84-4458-85　UCP2 100ug　377649

特徴

• Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP).
• UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak.
• UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane.
• They also reduce the mitochondrial membrane potential in mammalian cells.
• Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known.
• UCPs contain the three homologous protein domains of MACPs.
• This gene is expressed in many tissues, with the greatest expression in skeletal muscle.
• It is thought to play a role in nonshivering thermogenesis, obesity and diabetes.
• Chromosomal order is 5'-UCP3-UCP2-3’.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.