84-4458-41　SPARC 100ug　377604

特徴

• SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene.
• The human SPARC gene is 26.5kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33.
• SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV).
• Furthermore, SPARC is a glycoprotein in the bone that binds sodium.
• It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation.
• SPARC also shows affinity for collagen in addition to bone mineral calcium.
• A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.