84-4458-33　Six3 100ug　377594

特徴

• Homeobox protein SIX3 is a protein that in humans is encoded by the SIX3 gene.
• This gene encodes a member of the sine oculishomeobox transcription factor family.
• The encoded protein plays a role in eye development.
• Mutations in SIX3 are the cause of a severe brain malformation, called holoprosencephaly type 2 (HPE2).
• In HPE2, the brain fails to separate into two hemispheres during early embryonic development, leading to eye and brain malformations, which result in serious facial abnormalities.
• A mutant zebrafish knockout model has been developed, in which the anterior part of the head was missing due to the atypical increase of Wnt1 activity.
• When injected with SIX3, these zebrafish embryos were able to successfully develop a normal forebrain.
• When SIX3 was turned off in mice, resulting in a lack of retina formation due to excessive expression of Wnt8b in the region where the forebrain normally develops.
• Both of these studies demonstrate the importance of SIX3 activity in brain and eye development.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.