84-4458-29　SCF 100ug　377590

特徴

• Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117).
• The SCF gene is mapped to 12q21.32.
• SCF can exist both as a transmembrane protein and a soluble protein.
• This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis.
• SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors.
• The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.
• One of the problems of injecting SCF for therapeutic purposes is that SCF activates mast cells.
• The injection of SCF has been shown to cause allergic-like symptoms and the proliferation of mast cells and melanocytes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.