84-4458-26　RPS6 (40S Ribosomal Protein S6) 100ug　377587

特徴

• Ribosomal protein S6 (rpS6) is a component of the 40S ribosomal subunit and is therefore thought to be involved in regulating translation.
• Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit.
• Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.
• This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit.
• The protein belongs to the S6E family of ribosomal proteins.
• It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases.
• Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens.
• Dephosphorylation occurs at growth arrest.
• The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA.
• As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
• While the true function of rpS6 is currently under investigation, studies have shown that it is involved in the regulation of cell size, cell proliferation, and glucose homeostasis.
• Applications:Suitable for use in Western Blot, Immunohistohemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.