RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1.
This gene is mapped to chromosome 1p36.13 based on an alignment of the RNF186 sequence with the genomic sequence (GRCh38).
Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1.
Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human RNF186.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human RNF186 (Position: R86-L159) expressed in E. coli.
