84-4457-98　PGF 100ug　377560

特徴

• Placental growth factor (PGF, also known as PLGF) codes for an angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF).
• It is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro.
• PGF is located on chromosome 14 and has been conserved in evolution.
• It belongs to the family of vascular endothelial growth factors (VEGFs) and binds to the flt-1 VEGF receptor.
• PLGF-2, which is a PLGF isoform, binds neuropilin-1 and 2 in a heparin-dependent manner.
• PGF regulates inter- and intra molecular cross talk between the VEGF RTKs Flt1 and Flk1 and stimulates the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes.
• Moreover, it plays an important role in pathological angiogenic events and with exerting its biological activities through binding to VEGFR1.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.