In studies of the NDRG gene family in the human, novel mouse sequences of Ndr2 and Ndr3 were used to search the human genome databases.
This confirmed the existence of a human NDRG3 gene, homologous to mouse Ndr3.
The 3 known human NDRG proteins show considerable homology, with 54% amino acid sequence identity between NDRG1 and NDRG2, 67% between NDRG1 and NDRG3, and 58% between NDRG2 and NDRG3.
The NDRG3 gene was represented by 86 ESTs and a genomic clone from chromosome 20q11.21-q11.23.
This provisional chromosomal localization was confirmed by electronic PC.
Applications:Suitable for use in Western Blot, Immunohistohemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human NDRG3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NDRG3 (Position: L181-R239), expressed in E. coli. Species sequence homology: mouse (93.1%), rat (94.9%).
