GSTT1(glutathione S-transferase theta 1), also known as GST class-theta-1, Glutathione transferase T1-1, is an enzyme that in humans is encoded by the GSTT1 gene.
It is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds.
Human theta-class GST, termed GSTT1 has 239aa GSTT1 protein shares 80% sequence identity with the rat homolog.
Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta.
The theta class includes GSTT1 and GSTT2.
The GSTT1 and GSTT2 share 55% aa sequence identity and both of them were claimed to have an important role in human carcinogenesis.
The GSTT1 gene is located approximately 50kb away from the GSTT2 gene.
The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human GSTT1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GSTT1 (Position: L3-L231), expressed in E. coli.
