84-4455-97　ERCC1 100ug　377347

特徴

• DNA excision repair protein ERCC-1 is a protein that in humans is encoded by the ERCC1 gene.
• The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin.
• The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion.
• The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks.
• Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis.
• Multiple transcript variants encoding different isoforms have been found for this gene.
• The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.