This gene encodes a member of the epidermal growth factor superfamily.
The encoded preproprotein is proteolytically processed to generate the 53aa epidermal growth factor peptide.
This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types.
Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor.
Defects in this gene are the cause of hypomagnesemia type 4.
Dysregulation of this gene has been associated with the growth and progression of certain cancers.
Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Applications:Suitable for use in ELISA, Western Blot, Immunohistohemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes rat EGF.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat EGF (Position: N974-R1026), expressed in E. coli. Species sequence homology: human (69.8%), rmouse (77.4%).
