84-4455-77　DDX3 100ug　377326

特徴

• ATP-dependent RNA helicase DDX3X is an enzyme that in humans is encoded by the DDX3X gene.
• The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity.
• This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA.
• This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm.
• Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export.
• In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication.
• Misregulation of this gene has been implicated in tumorigenesis.
• This gene has a paralog located in the nonrecombining region of the Y chromosome.
• Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.