C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes.
It is composed of 5 identical, 21,500-molecular weight subunits.
CRP mediates activities associated with preimmune nonspecific host resistance.
CRP shows the strongest association with cardiovascular events.
It is detectable on the surface of about 4% of normal peripheral blood lymphocytes.
Acute phase reactant CRP is produced in the liver.
Applications:Suitable for use in Western Blot, Immunohistohemistry and ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity:Recognizes mouse Crp. Species Crossreactivity: rat
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Mo Rt
Immunogen:Recombinant protein corresponding to mouse Crp (Position: H20-S225), expressed in E. coli. Species sequence homology: human (71.9%), rat (72.7%).