CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene.
It is mapped to 11q23.3.
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.
This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11.
The epsilon polypeptide plays an essential role in T-cell development.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes mouse CD3 epsilon. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to mouse CD3 epsilon (Position: D22-D108) expressed in E. coli.
