Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene.
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family.
Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells.
This gene is also a candidate tumor suppressor gene for human breast cancer.
Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity:Recognizes rat Cardiac FABP. Species Crossreactivity: mouse and human
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Recombinant protein corresponding to rat Cardiac FABP (Position: A2-A133), expressed in E. coli. Species sequence homology: human and mouse