Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases.
The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins.
The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.
This gene encodes the large subunit of the ubiquitous enzyme, calpain 2.
Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.
Applications:Suitable for use in Western Blot, Immunohistohemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human Calpain 2. Species Crossreactivity: rat and mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Calpain 2 (Position: R500-L700), expressed in E. coli. Species sequence homology: mouse (93.5%), rat (92.5%).
