84-4455-11　Bcl-XL (Bcl-2-like Protein 1) 100ug　377254

特徴

• Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene.
• The protein encoded by this gene belongs to the BCL-2 protein family.
• BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.
• The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening.
• VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis.
• Alternative splicing results in multiple transcript variants encoding two different isoforms.
• The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
• Applications:Suitable for use in Western Blot, Immunohistohemistry.
• Other applications not tested.
• Recommended Dilution:lWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.