ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, is an enzyme that in humans is encoded by the ADAMTS4 gene.
ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases.
The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis.
The enzyme encoded by this gene lacks a C-terminal TS motif.
It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein.
The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffn): 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human ADAMTS4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa813-837, TPQDWLHRRAQILEILRRRPWAGRK from human ADAMTS4 at C-terminal, different from the related mouse sequence 5aa and from the related rat sequence by 6aa.
