84-4454-75　ADAM17 100ug　377211

特徴

• ADAM17(ADAM metallopeptidase domain 17), also called TACE (tumor necrosis factor-alpha-converting enzyme), is a 70kD enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases.
• Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form.
• Northern blot analysis revealed that the gene was expressed as a 5kb mRNA in all tissues examined.
• ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-alpha) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network.
• This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha), and is of known physiological importance.
• ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.