Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins.
It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins.
Mature human Brevican is 889 amino acids in length.
It contains multiple domains, with an N-terminal V-type Ig-like domain (aa 52-139) and a C-terminal EGF-like (aa 646-682) and C-type lectin domain (aa 695-802).
Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa 649-911 that serves as a GPI-anchor motif.
Over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes human Brevican.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp23-Pro911 from human Brevican expressed in NS0 cells. Swiss/UniProt Accession: Q96GW7
