The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubules.
Globular tubulin subunits are the building blocks of a microtubule, and the alpha/beta tubulin heterodimer forms the tubulin subunit, which is present in all eukaryotic cells.
gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubules.
Many cell movements, including beating of cilia and flagella, transport of membrane vesicles in the cytoplasm, alignment of chromosomes during meiosis and mitosis and migration of nerve-cell axons by extending the neuronal growth cone, are mediated by microtubules.
These movements are either the result of polymerization and depolymerization or the actions of microtubule motor proteins (1).
Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunohistochemistry (Paraffin): 1:100Immunofluorescence (IF-IC): 1:50Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and Peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human b-tubulin, and does not cross-react with recombinant alpha-tubulin. Species Crossreactivity: mouse, rat, monkey, bovine, zebrafish.
Isotype：IgG
Calc Applications Abbrev：FC IF IHC WB
Calc Crossreactivity：Bo Hu Mo Rt Ze
Immunogen：Synthetic peptide corresponding to the sequence of human beta-tubulin. Species sequence homology: xenopus
