Transforming growth factor-b (TGF-b) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4).
TGF-beta elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII).
Type I and type II receptors are serine/threonine kinases that form a heteromeric complex.
In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and thus activation of the type I receptor kinases (5).
The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-beta with high affinity but lacks a cytoplasmic signaling domain (6,7).
Expression of the type III receptor can regulate TGF-b signaling through presentation of the ligand to the signaling complex.
The only known direct TGF-beta signaling effectors are the Smads, which function by transducing signals from the cell surface directly to the nucleus and regulating transcription of target genes (8,9).
There are three isoforms of TGF-beta designated TGF-beta1, TGF-beta2, and TGF-beta3, which are encoded by distinct genes and are expressed in a tissue specific manner (10).
Each of the isoforms are synthesized as larger precursor proteins containing a propeptide region which gets cleaved prior to secretion from the cell.
Mature TGF-beta contains two polypeptides linked linked by disulfide bonds forming a protein of about 25 kD.
Applications: Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and Peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes recombinant TGF-beta1, TGF-beta2, and TGF-beta3. Also detects endogenous levels of the TGF-beta1 precursor proteins. Species Crossreactivity: human, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a region in the C-terminal of TGF-beta1.
