84-4454-61　Caveolin-1 (CAV1) 100ul　376939

特徴

• The 21-24kD integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae.
• Three members of the caveolins family (caveolin-1, -2 and -3) have been identified with different tissue distributions.
• Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1).
• Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2).
• Caveolins also interact with multiple signaling molecules, such as G-a subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2).
• It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction.
• Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5).
• Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
• Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence (IF-IC): 1:400Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Immunohistochemistry (paraffin): 1:250Immunohistochemistry (frozen): 1:1000Flow Cytometry: 1:50Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.