Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1).
It is implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3).
Casein kinase 1 phosphorylates beta-catenin on Ser45.
This phosphorylation event primes b-catenin for subsequent phosphorylation by GSK-3 (4-6).
GSK-3b destabilizes b-catenin by phosphorylating it at Ser33, 37 and Thr41 (7).
Mutations in these phosphorylation sites which result in the stabilization of beta-catenin protein levels have been found in many tumor cell lines (8).
Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Immunohistochemistry (Paraffin): 1:1600Immunohistochemistry (Frozen): 1:400ChIP: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and Peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human beta-catenin. Species Crossreactivity: human, mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：ChIP IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal of human beta-catenin. Species sequence homology: xenopus, chicken, porcine, bovine, canine, equine.
