84-4454-59　C/EBPbeta, NT (CCAAT/Enhancer-Binding Protein beta, NFIL6) 100ul　376934

特徴

• CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1).
• Six members of the family have been characterized (C/EBPalpha, -delta, -gamma, -epsilon and -zeta) and are distributed in a variety of tissues (1).
• There are two forms of C/EBPb, the 38kD liver activating protein (LAP) and the 20kD liver inhibitory protein (LIP) which may be products of alternative translation.
• The 38kD LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPb transcriptional activity (2).
• Phosphorylation of C/EBPb at distinct sites stimulates its transcriptional activity (3-5).
• Phosphorylation at Ser105 of rat C/EBPb, a unique site only present in the rat sequence, seems essential for rat C/EBPb activation (6).
• Applications: Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.