84-4452-70　ACLY (ATP Citrate Synthase, ATP-citrate Lyase) 100ug　370876

特徴

• ATP citrate lyase, also known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis.
• ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues.
• The enzyme is a tetramer of apparently identical subunits.
• The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.
• It is activated by insulin.
• In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.
• In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
• Applications: Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.