Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1).
Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a C-terminal tail (2).
Expression of vinculin were shown to be affected by the level of actin expression (2,3).
Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4).
Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin
Specificity：Recognizes human Vinculin. Species Crossreactivity: chicken, mouse, rat, rabbit
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Hu Mo Rb Rt
Immunogen：Synthetic peptide corresponding to 23aa of human Vinculin at N-terminal.
