Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
His-tag is one of the more popular epitope tags for expressed recombinant proteins.
Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ul/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes N-terminal or C-terminal His-tag recombinant proteins.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Immunogen：Synthetic peptide corresponding to HHHHHH of His-tag.
