TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts.
TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors.
Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2.0ug/mlWestern Blot: 0.1-0.2ug/mlImmunohistochemistry (paraffin): requires enzyme mediated antigen retrieval prior to staining of parrafin sections.
Proteinase K is recomended for this purpose
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human TIMP-1.
Isotype：IgG
Immunogen：Recombinant protein corresponding to human TIMP-1.
