Single Ig IL-1-related receptor (SIGIRR), a member of the Toll-like receptor-interleukin 1 receptor superfamily, defined by an intracellular Toll-IL-1R (TIR) domain.
SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signalling pathways.
It interacts with TLR4, TLR5, TLR9 and IL-1R through the extracellular Ig domain, or the intracellular TIR domain.
The Ig domain interferes with the heterodimerisation of IL-1R while the intracellular TIR domain inhibits both IL-1 and LPS signalling by attenuating the recruitment of receptor-proximal signalling components to TLR4.
SIGIRR is expressed in kidney, colon, small intestine, lung, spleen and liver.
It is not expressed in brain and muscle.
Applications:Suitable for use in Immunohistochemistry (frozen) and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen): 10ug/mlWestern Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse SIGIRR at ~43kD in A549 cell lysate. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：A peptide corresponding to 15aa at the C-terminus of mouse SIGIRR.
