SUMO-1 is an 11kD protein which structurally related to ubiquitin, and is added to proteins as a form of post-translational modification known as sumoylation.
Sumoylation can be cell cycle regulated, stimulated upon stress or induced by DNA damage, and binding elicits individual effects for each protein.
Effects include changes to protein stability, nuclear-cytosolic transport, and transcriptional regulation (mostly transcriptional repression).
Alternate transcriptional splice variants encoding different isoforms have been characterized.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes an epitope within the N-terminal region of human small ubiquitin-related modifier-1. Species Crossreactivity: rat, mouse.
Isotype：IgG
Immunogen：14aa peptide sequence near the N-terminal of human SUMO-1.
