PS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), is a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex.
VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation.
Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion.
PS34 has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/ml, detects a band of ~105kD in HEK-293T cell lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PIK3C3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa22-34 of human PIK3C3, KLH conjugated.
