ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kD multi-pass membrane protein belonging to the ORAI family.
Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels.
CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC.
ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor.
Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: detects a band of approximately 49kD in 49 cell lysates
Optimal dilution to be determined by researcher.
Recommended Positive Control:Human Ovary Total Protein Lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human ORAI1. Species Crossreactivity: mouse, rat. Not expected to cross react with either ORAI2 or ORAI3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 16 amino acid peptide from near the carboxy terminus of human ORAI1.
