Calcitonin receptor is a membrane protein with seven transmembrane domains that are coupled to G protein messenger systems.
The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts.
It is also expressed in a number of tissues during the development of the embyro.
It may be important in fetal morphogenesis.
Applications:Suitable for use in Immunohistochemistry, FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Paraffin): The use of 4% paraformaldehyde fixation, rather than formalin fixation, is recommended.
A sensitive detection system is also recommended; e.g.
a catalyzed signal amplification system.
Paraffin sections do not require protein digestion pre-treatment.
Does not require antigen retrieval using heat treatment prior to staining
Western Blot: Reducing conditionsOptimal working dilutions to be determined by researcher.
Recommended Positive Control Tissue:IHC: Kidney, cultered osteoclasts
Recommended Secondary Reagent:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Specificity：Recognizes rat calcitonin receptor, an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms. Species Crossreactivity: Human and mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a C-terminal sequence of rat calcitonin receptor.
