Wilms tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminal and a proline/glutamine-rich DNA-binding domain at the N-terminal.
It has an essential role in the normal development of the urogenital system, and it is mutated in a subset of patients with Wilms' tumor, the gene's namesake.
Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized.
There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.The WT1 protein has been found to bind a host of cellular factors, e.g.
p53, a known tumor suppressor.
Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human WT1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Leu310-Leu449 from human WT1, expressed in E. coli.
